Client:
Leading European Academic Research institute

Challenge:

Predicting the disease physiology in humans, given results from a whole genome RNAi screen for the disease phenotype in Drosophila melanogaster.
    
Strand's Solution:

We applied the following analysis techniques to dissect the pathways underlying the disease phenotype:

• Select candidate hits from the RNAi screen
• GO analysis to understand the gene functions 
• Significance analysis against all known KEGG pathways to understand underlying biological processes
• Ortholog mapping between Drosophila, mouse and humans
• Comparison of knock-out phenotypes in mouse and RNAi hits in Drosophila
• Building a protein interactions network with the "hits", and enriching with first degree neighbors, for the disease phenotype.  
• GO and KEGG analysis for the mouse and human orthologues of the disease genes in Drosophila
• Network centrality measures to identify key control points in the Protein protein interactions network